Three sodium metaphosphate crosslinking hyaluronic acid gel

Three sodium metaphosphate crosslinking hyaluronic acid gel
Hyaluronic acid oxide/collagen composite hydrogel scaffold preparation:

With sodium periodate to modification of hyaluronic acid, collagen and the neutral reaction of 37 ℃ to form water gel. Dimethyl aminobenzoic hyaluronic acid and formaldehyde reaction test TNBS method of collagen and hyaluronic acid oxidation degree of crosslinking. Cartilage cell embedding in the hydrogel culture and observe their growth state. Results hyaluronic acid oxidation were fixed in the hydrogel, collagen amino and oxidation aldehyde group of hyaluronic acid to form schiff base structure, the crosslinking degree increase with the increase of oxidation of hyaluronic acid content. Uniform distribution of chondrocytes in the hydrogel and activity is good, the addition of hyaluronic acid played a certain role to promote cell growth. Conclusion the collagen/hyaluronic acid oxide composite hydrogel bionic scaffold in cartilage tissue engineering has a great application prospect.

Three sodium metaphosphate crosslinking hyaluronic acid gel preparation:

Hyaluronic acid through crosslinking agent three sodium metaphosphate crosslinking reaction in alkaline solution, and then swelling gel cut into small pieces, soak in the acid solution, to its volume, after a quick colorless to light yellow, transparent, no particles cross-linked hyaluronic acid hydrogel. The invention of the crosslinked gel preparation technology is simple, low production cost, high yield, easy to enlarge production, and the gel has good moisturizing effect and silky feeling, and not sticky greasy, can be used in various fields.